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Exposure of filtration equipment to UV light is not adequate to ensure sterilization.
Apha standard methods 21st edition pdf series#
See paragraph (c) of this section.ģ Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and false-negative rate for total coliforms, using lactose broth, is less than 10 percent.Ĥ All filtration series must begin with membrane filtration equipment that has been sterilized by autoclaving. Laboratories should be careful to use only the approved versions of the methods, as product package inserts may not be the same as the approved versions of the methods.Ģ Incorporated by reference. The methods listed are the only versions that may be used for compliance with this rule. For vendor methods, the date of the method listed in paragraph (c) of this section is the date/version of the approved method. The methods listed are the only online versions that may be used. For the Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. For Standard Methods, either editions, 20th (1998) or 21st (2005), may be used. 21st ed.)ġ The procedures must be done in accordance with the documents listed in paragraph (c) of this section. 21st ed.).Įscherichia coli Procedure (following Lactose Fermentation Methods) Standard Total Coliform Membrane Filter Procedure Standard Methods Online 9221 D.1, D.2-99. Standard Methods Online 9221 B.1, B.2-99. Standard Total Coliform Fermentation Technique
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coli analyses in accordance with one of the analytical methods in the following table or one of the alternative methods listed in Appendix A to subpart C of part 141. (5) Systems must conduct total coliform and E. Dechlorination procedures are addressed in Section 9060A.2 of Standard Methods for the Examination of Water and Wastewater (20th and 21st editions). (4) If water having residual chlorine (measured as free, combined, or total chlorine) is to be analyzed, sufficient sodium thiosulfate (Na 2S 2O 3) must be added to the sample bottle before sterilization to neutralize any residual chlorine in the water sample. Systems are encouraged but not required to hold samples below 10 deg. (3) The time from sample collection to initiation of test medium incubation may not exceed 30 hours. coli a determination of density is not required. (2) Systems need only determine the presence or absence of total coliforms and E.
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(1) The standard sample volume required for analysis, regardless of analytical method used, is 100 ml. § 141.852 Analytical methods and laboratory certification.